The Mechanism of Selective Kinesin Inhibition by Kinesin Binding Protein
نویسندگان
چکیده
منابع مشابه
Cayman ataxia protein caytaxin is transported by kinesin along neurites through binding to kinesin light chains.
Deficiency of caytaxin results in hereditary ataxia or dystonia in humans, mice and rats. Our yeast two-hybrid screen identified kinesin light chains (KLCs) as caytaxin-binding proteins. The tetratricopeptide-repeat region of KLC1 recognizes the ELEWED sequence (amino acids 115-120) of caytaxin. This motif is conserved among BNIP-2 family members and other KLC-interacting kinesin cargo proteins...
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We have prepared and characterized seven mouse monoclonal antibodies (SUK 1-7) to the 130-kD heavy chain of sea urchin egg kinesin. On immunoblots, SUK 3 and SUK 4 cross-reacted with Drosophila embryo 116-kD heavy chains, and SUK 4, SUK 5, SUK 6, and SUK 7 bound to the 120-kD heavy chains of bovine brain kinesin. Three out of seven monoclonal antikinesins (SUK 4, SUK 6, and SUK 7) caused a dose...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2021
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2020.11.279